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mouse macrophage colony  (R&D Systems)


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    R&D Systems mouse macrophage colony
    Mouse Macrophage Colony, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 879 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+m+csf+protein/Recombinant+Mouse+M-CSF+Protein/pm42778106-205-0-17
    Average 96 stars, based on 879 article reviews
    mouse macrophage colony - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Osteotropic properties of mesenchymal stromal cells generated in Masquelet's induced membrane technique.
    Article Snippet: From the cell suspension, macrophages were isolated by density gradient centrifugation using a LymphoprepTM solution (AXIS-SHIELD, Oslo, Norway). .. The isolated mouse bone marrow macrophages were cultured in the medium described above and further supplemented with 30 ng/mL of recombinant mouse M-CSF protein (416-ML, R&D Systems, Minneapolis, MN, USA). ..

    Article Title: DcR3 reprograms macrophage plasticity to promote wound healing and hair regeneration.
    Article Snippet: Background: Macrophages are key regulators of tissue repair, with M2-polarized subsets promoting antiinflammatory, pro-regenerative functions.. Decoy Receptor 3 (DcR3), a soluble TNF receptor superfamily member, modulates macrophage polarization, but its role in cutaneous regeneration remains unclear.. Objective: To elucidate the immunomodulatory role of DcR3 in macrophage polarization and evaluate its therapeutic potential in skin wound repair and hair follicle regeneration.

    Article Title: Osteotropic properties of mesenchymal stromal cells generated in Masquelet’s induced membrane technique
    Article Snippet: From the cell suspension, macrophages were isolated by density gradient centrifugation using a LymphoprepTM solution (AXIS-SHIELD, Oslo, Norway). .. The isolated mouse bone marrow macrophages were cultured in the medium described above and further supplemented with 30 ng/mL of recombinant mouse M-CSF protein (416-ML, R&D Systems, Minneapolis, MN, USA). ..

    Cell Culture:

    Article Title: Osteotropic properties of mesenchymal stromal cells generated in Masquelet's induced membrane technique.
    Article Snippet: From the cell suspension, macrophages were isolated by density gradient centrifugation using a LymphoprepTM solution (AXIS-SHIELD, Oslo, Norway). .. The isolated mouse bone marrow macrophages were cultured in the medium described above and further supplemented with 30 ng/mL of recombinant mouse M-CSF protein (416-ML, R&D Systems, Minneapolis, MN, USA). ..

    Article Title: DcR3 reprograms macrophage plasticity to promote wound healing and hair regeneration.
    Article Snippet: Background: Macrophages are key regulators of tissue repair, with M2-polarized subsets promoting antiinflammatory, pro-regenerative functions.. Decoy Receptor 3 (DcR3), a soluble TNF receptor superfamily member, modulates macrophage polarization, but its role in cutaneous regeneration remains unclear.. Objective: To elucidate the immunomodulatory role of DcR3 in macrophage polarization and evaluate its therapeutic potential in skin wound repair and hair follicle regeneration.

    Article Title: Osteotropic properties of mesenchymal stromal cells generated in Masquelet’s induced membrane technique
    Article Snippet: From the cell suspension, macrophages were isolated by density gradient centrifugation using a LymphoprepTM solution (AXIS-SHIELD, Oslo, Norway). .. The isolated mouse bone marrow macrophages were cultured in the medium described above and further supplemented with 30 ng/mL of recombinant mouse M-CSF protein (416-ML, R&D Systems, Minneapolis, MN, USA). ..

    Recombinant:

    Article Title: Osteotropic properties of mesenchymal stromal cells generated in Masquelet's induced membrane technique.
    Article Snippet: From the cell suspension, macrophages were isolated by density gradient centrifugation using a LymphoprepTM solution (AXIS-SHIELD, Oslo, Norway). .. The isolated mouse bone marrow macrophages were cultured in the medium described above and further supplemented with 30 ng/mL of recombinant mouse M-CSF protein (416-ML, R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Injectable magnesium-bisphosphonate MOF-based bone adhesive prevents excessive fibrosis for osteoporotic fracture repair
    Article Snippet: .. Gelatin (V900863, Merck, Germany); 2-chlorethylamine hydrochloride (C107599, aladdin, China); Hydrochloric acid (H399657, Aladdin, China); 1-ethyl-(3-dimethylaminopropyl) carbamide diimide (EDC, E106172, aladdin, China); N-hydroxythiosuccinimide (Sulfo-NHS, H109337, aladdin, China); 3, 4-dihydroxyphenylacetic acid (D106475, aladdin, China); Starch from maize (aladdin, A120984); Sodium periodate (S104091, aladdin, China); Rhodamine B (R104960, Aladdin, China); Low-glucose dulbecco’s modified eagle’s medium (DMEM), α-minimum essential medium (α-MEM), fetal bovine serum (FBS), penicillin–streptomycin, trypsin and phosphate-buffered saline (PBS) were obtained from Gibco (USA); Dexamethasone (≥97%, D4902, Merck, Germany); Ascorbic acid (A4403, Merck, Germany); Sodium β-glycerophosphate (G9422, Merck, Germany); L -proline (P5607, Merck, Germany); Trisodium L -ascorbate-2-phosphate salt (49752, Merck, Germany); bFGF (HY-P7091, MedChemExpress, USA); Alizarin Red S (A5533, Merck, Germany); Sodium phosphate (S113693, Aladdin, China); Hexadecylpyridinium chloride (C129534, Aladdin, China); Recombinant mouse M-CSF protein (416-ML, R&D Systems, USA); Recombinant mouse TRANCE/RANKL/TNFSF11 ( E. coli -expressed, 462-TEC, R&D Systems, USA); Bovine Serum Albumin (BSA, A1933, Merck, Germany) Calcein (C0875, Merck, Germany); Alizarin 3-methyl-amino-diacetic acid (A3882, Merck, Germany). ..

    Article Title: Liquiritigenin suppresses osteoclastogenesis via multi-target mechanisms involving NF-κB/PI3K-AKT signaling pathways and metabolic reprogramming.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: DcR3 reprograms macrophage plasticity to promote wound healing and hair regeneration.
    Article Snippet: Background: Macrophages are key regulators of tissue repair, with M2-polarized subsets promoting antiinflammatory, pro-regenerative functions.. Decoy Receptor 3 (DcR3), a soluble TNF receptor superfamily member, modulates macrophage polarization, but its role in cutaneous regeneration remains unclear.. Objective: To elucidate the immunomodulatory role of DcR3 in macrophage polarization and evaluate its therapeutic potential in skin wound repair and hair follicle regeneration.

    Article Title: Injectable magnesium-bisphosphonate MOF-based bone adhesive prevents excessive fibrosis for osteoporotic fracture repair.
    Article Snippet: .. Gelatin (V900863,Merck, Germany); 2-chlorethylamine hydrochloride (C107599, aladdin, China); Hydrochloric acid (H399657, Aladdin, China); 1-ethyl-(3-dimethylaminopropyl) carbamide diimide (EDC, E106172, aladdin, China); N-hydroxythiosuccinimide (Sulfo-NHS, H109337, aladdin, China); 3, 4-dihydroxyphenylacetic acid (D106475, aladdin, China); Starch from maize (aladdin, A120984); Sodium periodate (S104091, aladdin, China); Rhodamine B (R104960, Aladdin, China); Low-glucose dulbecco’s modified eagle’s medium (DMEM), αminimum essential medium (α-MEM), fetal bovine serum (FBS), penicillin–streptomycin, trypsin and phosphate-buffered saline (PBS) were obtained from Gibco (USA); Dexamethasone (≥97%, D4902, Merck, Germany); Ascorbic acid (A4403, Merck, Germany); Sodium βglycerophosphate (G9422, Merck, Germany); L-proline (P5607, Merck, Germany); Trisodium L-ascorbate-2-phosphate salt (49752, Merck, Germany); bFGF (HY-P7091, MedChemExpress, USA); Alizarin Red S (A5533, Merck, Germany); Sodium phosphate (S113693, Aladdin, China); Hexadecylpyridinium chloride (C129534, Aladdin, China); Recombinant mouse M-CSF protein (416-ML, R&D Systems, USA); Recombinant mouse TRANCE/RANKL/TNFSF11 (E. coli-expressed, 462- TEC, R&D Systems, USA); Bovine Serum Albumin (BSA, A1933, Merck, Germany) Calcein (C0875, Merck, Germany); Alizarin 3-methyl-aminodiacetic acid (A3882, Merck, Germany). ..

    Article Title: Osteotropic properties of mesenchymal stromal cells generated in Masquelet’s induced membrane technique
    Article Snippet: From the cell suspension, macrophages were isolated by density gradient centrifugation using a LymphoprepTM solution (AXIS-SHIELD, Oslo, Norway). .. The isolated mouse bone marrow macrophages were cultured in the medium described above and further supplemented with 30 ng/mL of recombinant mouse M-CSF protein (416-ML, R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Isonardosinone suppresses osteoclastogenesis through TRAF2/TRAF6-mediated disruption of calcium signaling and coordinate inhibition of MAPK/NF-κB pathways
    Article Snippet: Penicillin-streptomycin solution (P/S, Cat #15,140,122), fetal bovine serum (FBS, Cat #10099141C), minimum essential medium α (MEM-α, Cat #12,571,063), and trypsin (Cat #25,200,056) were obtained from Thermo Fisher Scientific (China) Limited (Shanghai, China). .. Recombinant mouse TRANCE/RANKL (Cat #462-TEC-010) and recombinant mouse M-CSF protein (Cat #416-ML-050) were sourced from R&D Systems (Shanghai, China). .. DAPI (Cat #C0065), TRITC phalloidin (Cat #CA1610), reactive oxygen species assay kit (Cat #CA1410), Fluo-4 AM (Cat #F8500), CCK-8 cell proliferation and cytotoxicity assay kit (Cat #CA1210), and tartrate-resistant acid phosphatase (TRAP) stain kit (Cat #G1492) were purchased from Solarbio Technology Co., Ltd. (Beijing, China).

    Article Title: Nuclear pore complex-mediated chromatin reorganization regulates the epigenetic landscape of osteoclasts during differentiation
    Article Snippet: 91 92 Materials and methods 93 Reagents 94 RAW264.7 cell line, Dulbecco's modified Eagle medium (DMEM), α-minimum 95 essential medium (α-MEM), fetal bovine serum, and penicillin-streptomycin were 96 obtained from Procell Life Science (Wuhan, China). .. Recombinant mouse RANKL 97 (462-TR-MTO-100UG) and recombinant mouse M-CSF protein (455-MC-050) were 98 purchased from R&D Systems (Minneapolis, Minnesota, USA). .. The TRAP staining 99 kit was obtained from Sigma-Aldrich (New York, USA).

    Starch:

    Article Title: Injectable magnesium-bisphosphonate MOF-based bone adhesive prevents excessive fibrosis for osteoporotic fracture repair
    Article Snippet: .. Gelatin (V900863, Merck, Germany); 2-chlorethylamine hydrochloride (C107599, aladdin, China); Hydrochloric acid (H399657, Aladdin, China); 1-ethyl-(3-dimethylaminopropyl) carbamide diimide (EDC, E106172, aladdin, China); N-hydroxythiosuccinimide (Sulfo-NHS, H109337, aladdin, China); 3, 4-dihydroxyphenylacetic acid (D106475, aladdin, China); Starch from maize (aladdin, A120984); Sodium periodate (S104091, aladdin, China); Rhodamine B (R104960, Aladdin, China); Low-glucose dulbecco’s modified eagle’s medium (DMEM), α-minimum essential medium (α-MEM), fetal bovine serum (FBS), penicillin–streptomycin, trypsin and phosphate-buffered saline (PBS) were obtained from Gibco (USA); Dexamethasone (≥97%, D4902, Merck, Germany); Ascorbic acid (A4403, Merck, Germany); Sodium β-glycerophosphate (G9422, Merck, Germany); L -proline (P5607, Merck, Germany); Trisodium L -ascorbate-2-phosphate salt (49752, Merck, Germany); bFGF (HY-P7091, MedChemExpress, USA); Alizarin Red S (A5533, Merck, Germany); Sodium phosphate (S113693, Aladdin, China); Hexadecylpyridinium chloride (C129534, Aladdin, China); Recombinant mouse M-CSF protein (416-ML, R&D Systems, USA); Recombinant mouse TRANCE/RANKL/TNFSF11 ( E. coli -expressed, 462-TEC, R&D Systems, USA); Bovine Serum Albumin (BSA, A1933, Merck, Germany) Calcein (C0875, Merck, Germany); Alizarin 3-methyl-amino-diacetic acid (A3882, Merck, Germany). ..

    Article Title: Injectable magnesium-bisphosphonate MOF-based bone adhesive prevents excessive fibrosis for osteoporotic fracture repair.
    Article Snippet: .. Gelatin (V900863,Merck, Germany); 2-chlorethylamine hydrochloride (C107599, aladdin, China); Hydrochloric acid (H399657, Aladdin, China); 1-ethyl-(3-dimethylaminopropyl) carbamide diimide (EDC, E106172, aladdin, China); N-hydroxythiosuccinimide (Sulfo-NHS, H109337, aladdin, China); 3, 4-dihydroxyphenylacetic acid (D106475, aladdin, China); Starch from maize (aladdin, A120984); Sodium periodate (S104091, aladdin, China); Rhodamine B (R104960, Aladdin, China); Low-glucose dulbecco’s modified eagle’s medium (DMEM), αminimum essential medium (α-MEM), fetal bovine serum (FBS), penicillin–streptomycin, trypsin and phosphate-buffered saline (PBS) were obtained from Gibco (USA); Dexamethasone (≥97%, D4902, Merck, Germany); Ascorbic acid (A4403, Merck, Germany); Sodium βglycerophosphate (G9422, Merck, Germany); L-proline (P5607, Merck, Germany); Trisodium L-ascorbate-2-phosphate salt (49752, Merck, Germany); bFGF (HY-P7091, MedChemExpress, USA); Alizarin Red S (A5533, Merck, Germany); Sodium phosphate (S113693, Aladdin, China); Hexadecylpyridinium chloride (C129534, Aladdin, China); Recombinant mouse M-CSF protein (416-ML, R&D Systems, USA); Recombinant mouse TRANCE/RANKL/TNFSF11 (E. coli-expressed, 462- TEC, R&D Systems, USA); Bovine Serum Albumin (BSA, A1933, Merck, Germany) Calcein (C0875, Merck, Germany); Alizarin 3-methyl-aminodiacetic acid (A3882, Merck, Germany). ..

    Modification:

    Article Title: Injectable magnesium-bisphosphonate MOF-based bone adhesive prevents excessive fibrosis for osteoporotic fracture repair
    Article Snippet: .. Gelatin (V900863, Merck, Germany); 2-chlorethylamine hydrochloride (C107599, aladdin, China); Hydrochloric acid (H399657, Aladdin, China); 1-ethyl-(3-dimethylaminopropyl) carbamide diimide (EDC, E106172, aladdin, China); N-hydroxythiosuccinimide (Sulfo-NHS, H109337, aladdin, China); 3, 4-dihydroxyphenylacetic acid (D106475, aladdin, China); Starch from maize (aladdin, A120984); Sodium periodate (S104091, aladdin, China); Rhodamine B (R104960, Aladdin, China); Low-glucose dulbecco’s modified eagle’s medium (DMEM), α-minimum essential medium (α-MEM), fetal bovine serum (FBS), penicillin–streptomycin, trypsin and phosphate-buffered saline (PBS) were obtained from Gibco (USA); Dexamethasone (≥97%, D4902, Merck, Germany); Ascorbic acid (A4403, Merck, Germany); Sodium β-glycerophosphate (G9422, Merck, Germany); L -proline (P5607, Merck, Germany); Trisodium L -ascorbate-2-phosphate salt (49752, Merck, Germany); bFGF (HY-P7091, MedChemExpress, USA); Alizarin Red S (A5533, Merck, Germany); Sodium phosphate (S113693, Aladdin, China); Hexadecylpyridinium chloride (C129534, Aladdin, China); Recombinant mouse M-CSF protein (416-ML, R&D Systems, USA); Recombinant mouse TRANCE/RANKL/TNFSF11 ( E. coli -expressed, 462-TEC, R&D Systems, USA); Bovine Serum Albumin (BSA, A1933, Merck, Germany) Calcein (C0875, Merck, Germany); Alizarin 3-methyl-amino-diacetic acid (A3882, Merck, Germany). ..

    Article Title: Injectable magnesium-bisphosphonate MOF-based bone adhesive prevents excessive fibrosis for osteoporotic fracture repair.
    Article Snippet: .. Gelatin (V900863,Merck, Germany); 2-chlorethylamine hydrochloride (C107599, aladdin, China); Hydrochloric acid (H399657, Aladdin, China); 1-ethyl-(3-dimethylaminopropyl) carbamide diimide (EDC, E106172, aladdin, China); N-hydroxythiosuccinimide (Sulfo-NHS, H109337, aladdin, China); 3, 4-dihydroxyphenylacetic acid (D106475, aladdin, China); Starch from maize (aladdin, A120984); Sodium periodate (S104091, aladdin, China); Rhodamine B (R104960, Aladdin, China); Low-glucose dulbecco’s modified eagle’s medium (DMEM), αminimum essential medium (α-MEM), fetal bovine serum (FBS), penicillin–streptomycin, trypsin and phosphate-buffered saline (PBS) were obtained from Gibco (USA); Dexamethasone (≥97%, D4902, Merck, Germany); Ascorbic acid (A4403, Merck, Germany); Sodium βglycerophosphate (G9422, Merck, Germany); L-proline (P5607, Merck, Germany); Trisodium L-ascorbate-2-phosphate salt (49752, Merck, Germany); bFGF (HY-P7091, MedChemExpress, USA); Alizarin Red S (A5533, Merck, Germany); Sodium phosphate (S113693, Aladdin, China); Hexadecylpyridinium chloride (C129534, Aladdin, China); Recombinant mouse M-CSF protein (416-ML, R&D Systems, USA); Recombinant mouse TRANCE/RANKL/TNFSF11 (E. coli-expressed, 462- TEC, R&D Systems, USA); Bovine Serum Albumin (BSA, A1933, Merck, Germany) Calcein (C0875, Merck, Germany); Alizarin 3-methyl-aminodiacetic acid (A3882, Merck, Germany). ..

    Saline:

    Article Title: Injectable magnesium-bisphosphonate MOF-based bone adhesive prevents excessive fibrosis for osteoporotic fracture repair
    Article Snippet: .. Gelatin (V900863, Merck, Germany); 2-chlorethylamine hydrochloride (C107599, aladdin, China); Hydrochloric acid (H399657, Aladdin, China); 1-ethyl-(3-dimethylaminopropyl) carbamide diimide (EDC, E106172, aladdin, China); N-hydroxythiosuccinimide (Sulfo-NHS, H109337, aladdin, China); 3, 4-dihydroxyphenylacetic acid (D106475, aladdin, China); Starch from maize (aladdin, A120984); Sodium periodate (S104091, aladdin, China); Rhodamine B (R104960, Aladdin, China); Low-glucose dulbecco’s modified eagle’s medium (DMEM), α-minimum essential medium (α-MEM), fetal bovine serum (FBS), penicillin–streptomycin, trypsin and phosphate-buffered saline (PBS) were obtained from Gibco (USA); Dexamethasone (≥97%, D4902, Merck, Germany); Ascorbic acid (A4403, Merck, Germany); Sodium β-glycerophosphate (G9422, Merck, Germany); L -proline (P5607, Merck, Germany); Trisodium L -ascorbate-2-phosphate salt (49752, Merck, Germany); bFGF (HY-P7091, MedChemExpress, USA); Alizarin Red S (A5533, Merck, Germany); Sodium phosphate (S113693, Aladdin, China); Hexadecylpyridinium chloride (C129534, Aladdin, China); Recombinant mouse M-CSF protein (416-ML, R&D Systems, USA); Recombinant mouse TRANCE/RANKL/TNFSF11 ( E. coli -expressed, 462-TEC, R&D Systems, USA); Bovine Serum Albumin (BSA, A1933, Merck, Germany) Calcein (C0875, Merck, Germany); Alizarin 3-methyl-amino-diacetic acid (A3882, Merck, Germany). ..

    Article Title: Injectable magnesium-bisphosphonate MOF-based bone adhesive prevents excessive fibrosis for osteoporotic fracture repair.
    Article Snippet: .. Gelatin (V900863,Merck, Germany); 2-chlorethylamine hydrochloride (C107599, aladdin, China); Hydrochloric acid (H399657, Aladdin, China); 1-ethyl-(3-dimethylaminopropyl) carbamide diimide (EDC, E106172, aladdin, China); N-hydroxythiosuccinimide (Sulfo-NHS, H109337, aladdin, China); 3, 4-dihydroxyphenylacetic acid (D106475, aladdin, China); Starch from maize (aladdin, A120984); Sodium periodate (S104091, aladdin, China); Rhodamine B (R104960, Aladdin, China); Low-glucose dulbecco’s modified eagle’s medium (DMEM), αminimum essential medium (α-MEM), fetal bovine serum (FBS), penicillin–streptomycin, trypsin and phosphate-buffered saline (PBS) were obtained from Gibco (USA); Dexamethasone (≥97%, D4902, Merck, Germany); Ascorbic acid (A4403, Merck, Germany); Sodium βglycerophosphate (G9422, Merck, Germany); L-proline (P5607, Merck, Germany); Trisodium L-ascorbate-2-phosphate salt (49752, Merck, Germany); bFGF (HY-P7091, MedChemExpress, USA); Alizarin Red S (A5533, Merck, Germany); Sodium phosphate (S113693, Aladdin, China); Hexadecylpyridinium chloride (C129534, Aladdin, China); Recombinant mouse M-CSF protein (416-ML, R&D Systems, USA); Recombinant mouse TRANCE/RANKL/TNFSF11 (E. coli-expressed, 462- TEC, R&D Systems, USA); Bovine Serum Albumin (BSA, A1933, Merck, Germany) Calcein (C0875, Merck, Germany); Alizarin 3-methyl-aminodiacetic acid (A3882, Merck, Germany). ..

    Staining:

    Article Title: Liquiritigenin suppresses osteoclastogenesis via multi-target mechanisms involving NF-κB/PI3K-AKT signaling pathways and metabolic reprogramming.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Ex Vivo:

    Article Title: DcR3 reprograms macrophage plasticity to promote wound healing and hair regeneration.
    Article Snippet: Background: Macrophages are key regulators of tissue repair, with M2-polarized subsets promoting antiinflammatory, pro-regenerative functions.. Decoy Receptor 3 (DcR3), a soluble TNF receptor superfamily member, modulates macrophage polarization, but its role in cutaneous regeneration remains unclear.. Objective: To elucidate the immunomodulatory role of DcR3 in macrophage polarization and evaluate its therapeutic potential in skin wound repair and hair follicle regeneration.



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